How To Make Biuret Reagent

BIURET TEST

... O 0.75g Sodium potassium tartrate 0.3g Dissolve in 250cm 3 H 2 O Solution 2 Sodium hydroxide 15g Dissolve in 150cm 3 H 2 O Add 2 to 1 mixing thoroughly and make up to 500cm 3. If a precipitate forms 0.5g potassium iodide can be added Method • To perform the quantitative test add 2 cm 3 of Biuret reagent to 0.5 cm 3 of ...

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Determination of the Sensitivity Range of Biuret Test for ...

For the control, 1-mL of distilled water was added to 4.5-mL of biuret reagent in a test tube. For the test sample, 1-mL of the protein sample was added to 4.5-mL of biuret reagent.

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Biuret Protein Assay (BE-402B)

Mix equal volume of Biuret Reagent-I and Biuret Reagent-II to make Working Biuret Reagent just before the start of the experiment. Each student group needs 20ml Working Biuret Reagent.

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Laboratory 5 - Quantitative Biuret Analysis v2.5 revised 9/17/07

So, we must make a standard curve, to compare our unknown to. (make graph., draw tubes on board). We make a series of KNOWN samples, with equal amounts of biuret's reagent in each one, but different concentrations of BSA.

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CAHS 101L – General Biology Lab I – Laboratory #3

Protein - Biuret reagent - mix sample with one pipette of Biuret & mix Observations & Results: Make a TABLE to record your results: for each food, test for Sugar, Starch, Lipid, and Protein.

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Assays for Protein Quantification

Mix equal volume of Biuret Reagent-I and Biuret Reagent-II to make Working Biuret Reagent just before the start of the experiment. Each student group needs 20ml Working Biuret Reagent.

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Spectrophotometric Determination of Total Protein-Biuret Method

Procedure 1) Pipette 5.00mL of the biuret reagent into a each of 7 test tubes. 2) Pipette 5.00mL of the biuret blank reagent into a each of 7 test tubes.

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We compare results by this new method with those by a manual method in which trichloroacetic acid precipitation and biuret reagent are used. The new method shows good precision and excellent correlation (r = 0.997) with the manual method. The ease and convenience of this assay should make this a ...

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Identifying Macromolecules

Materials Test Tube Distilled Water Glass Marking Pencil Graduated Cylinder Apple Juice Test Tube Rack Sudan III Stain Pipette Biuret Reagent Potato Solution Hot Plate Beaker Oatmeal Solution Test Tube Holder Milk Benedict's Solution Iodine Cooking Oil Gelatin Solution Yogurt Procedure (GOGGLES MUST BE WORN FOR THE ...

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Lab 3. PROTEIN DETERMINATION

You can also make the 2 dilutions (10-1 and 10-2) of the unknown at this time. HAZARDS Biuret reagent is a strong base (pH >12) and toxic due to the copper.

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