Hbss Recipe

HBSS - HANK'S BALANCED SALT SOLUTION

HBSS_Formulation HBSS_Formulation. L IQUID Catalog Nos. 9220 9230 (10X) 9224 9228 9232 Component mg/L mg/L mg/L mg/L mg/L Sodium Chloride 8000 80000 8000 8000 8000 Potassium Chloride 400 4000 400 400 400 Potassium Phosphate, 60 600 60 60 60 monobasic KH 2 PO 4 Glucose 1000 10000 1000 1000 1000 ...

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The Slice Overlay Assay: A Versatile Tool to Study the ...

Transfer the slices as they are being cut to a new dish containing ice-cold Complete HBSS (Recipe 3) using a large-open ing flamed glass Pasteur pipette.

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Chapter 2 General methodology

... (0.25% trypsin .1mM EDTA-4Nain HBSS without Calcium or magnesium) GibcoBRL- In vitrogen corporation, Canada Williams media E powder With l-Glutamine, ...

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Newborn Screening ACT Sheet

... (HbSS Disease or HbS/Beta Zero Thalassemia) Sickle Cell Anemia Differential Diagnosis: Homozygous sickle cell disease (Hb SS), sickle beta-zero thalassemia, ...

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Antisickling activities of two ethnomedicinal plant recipes ...

The method employed involved the inhibition of sodium metabisulphite induced sickling of HbSS red blood cells, ... Extracts of Recipe 1 (consisting of 28 plants) and 2 (consisting of 7 plants) showed antisickling activities; ...

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Producing Hybrid Catfish Fry:

Place the testes (or pieces of testes) in a container containing HBSS (the recipe for HBSS is included in the appendix, this recipe works well for me, ...

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Isolation of Murine Natural Killer Cells

BASIC PROTOCOL PREPARATION OF NK CELLS FROM MOUSE SPLEEN Materials C57BL/6- or C57BL/10 mice Tris/NH 4 Cl lysing buffer (see recipe) HBSS/3% FBS (see recipe) ...

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Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation

Trypsin/EDTA solution Ca 2+ - and Mg 2+-free HBSS (see recipe) containing: 2.5 g/liter porcine trypsin (0.25%) 0.38 g/liter EDTA⋅4H 2 O Store aliquots up to 1 year at −20°C Aliquots can be thawed and stored at 4 ° C for up to 1 week.

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Basic Techniques for Mammalian Cell Tissue Culture

Materials Primary cultures of cells HBSS ... (see recipe), 37°C Complete medium with serum: e.g., supplemented DMEM (APPENDIX 2A) with 10% to 15% (v/v) FBS ...

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"Assessment of Lymphocyte Development in Radiation Bone ...

... (see recipe) Hanks'balanced salt solution (HBSS; APPENDIX 2A) containing 5% (v/v) newborn calf serum (CS) Fluorescein-labeled MAbA20.1 specific for the CD45 a ...

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